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ATCC material human melanoma cell lines c32
Material Human Melanoma Cell Lines C32, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/material human melanoma cell lines c32/product/ATCC
Average 95 stars, based on 305 article reviews

material human melanoma cell lines c32 - by Bioz Stars, 2026-06

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Schematic of e‐Medi‐Patch setup with plausible (a) mechanism of drug release; (b) Incubation of Medi‐patch (attached to sterilized tape) with cells for in‐vitro experiments (c) Bright field images of C32 cells after 48 h with free NIC and different formulations of medi‐patch exhibiting change in cellular morphology and growth density after 48 h treatment confirming slow and successful release of NIC over time. (d) C32 cells and (e) GR‐PCL incubated C32 cells showed negligible cell death [Hoechst33342 (blue) stains all cells, Calcein AM (green) stains live cells, Propidium iodide (red) stains dead cells] confirming insignificant effect of only GR‐PCL medi‐patch in absence of NIC (f) The % cell viability of C32 population with treatment groups post 48 and 72 h of incubation showed comparable efficiency of NIC, either released from the composites or as a free drug.

Journal: Advanced Healthcare Materials

Article Title: Electro‐Stimulated Graphene‐Polymer Nanocomposites Enable Wearable Patches With Feedback‐Controlled Drug Release

doi: 10.1002/adhm.202505894

Figure Lengend Snippet: Schematic of e‐Medi‐Patch setup with plausible (a) mechanism of drug release; (b) Incubation of Medi‐patch (attached to sterilized tape) with cells for in‐vitro experiments (c) Bright field images of C32 cells after 48 h with free NIC and different formulations of medi‐patch exhibiting change in cellular morphology and growth density after 48 h treatment confirming slow and successful release of NIC over time. (d) C32 cells and (e) GR‐PCL incubated C32 cells showed negligible cell death [Hoechst33342 (blue) stains all cells, Calcein AM (green) stains live cells, Propidium iodide (red) stains dead cells] confirming insignificant effect of only GR‐PCL medi‐patch in absence of NIC (f) The % cell viability of C32 population with treatment groups post 48 and 72 h of incubation showed comparable efficiency of NIC, either released from the composites or as a free drug.

Article Snippet: Human melanoma cell line C32 was obtained from American Type Culture Collection (ATCC).

Techniques: Incubation, In Vitro

Comparative Flow Cytometry analysis of cells incubated with Medi‐patch (CD44‐PE have been used as markers) for studying (a) control non‐cancerous b.End3 cells with negligible CD44(+) cell population; (b) C32 control population (c) GR‐PCL increases CD44 expression in C32 (d) loss of stemness property (CD44) in a significant C32 population (∼11%) treated with NIC‐GR‐PCL where effect of release NIC from e‐Medi‐Patch is confirmatory (e) Mechanistic roles of NIC in inhibiting the stem cell phenotype, survival, proliferation, migration in cancer cells (f) In MFI (mean fluorescent intensities) measurements b.End3 cells showed negligible CD44 expression without treatment which increases with GR‐PCL and decreases with NIC‐GR‐PCL medi‐patch (g) C32 showed gradual decrease in CD44 expression with NIC‐medi‐patch confirming efficacy of NIC‐GR‐PCL medi‐patch.

Journal: Advanced Healthcare Materials

Article Title: Electro‐Stimulated Graphene‐Polymer Nanocomposites Enable Wearable Patches With Feedback‐Controlled Drug Release

doi: 10.1002/adhm.202505894

Figure Lengend Snippet: Comparative Flow Cytometry analysis of cells incubated with Medi‐patch (CD44‐PE have been used as markers) for studying (a) control non‐cancerous b.End3 cells with negligible CD44(+) cell population; (b) C32 control population (c) GR‐PCL increases CD44 expression in C32 (d) loss of stemness property (CD44) in a significant C32 population (∼11%) treated with NIC‐GR‐PCL where effect of release NIC from e‐Medi‐Patch is confirmatory (e) Mechanistic roles of NIC in inhibiting the stem cell phenotype, survival, proliferation, migration in cancer cells (f) In MFI (mean fluorescent intensities) measurements b.End3 cells showed negligible CD44 expression without treatment which increases with GR‐PCL and decreases with NIC‐GR‐PCL medi‐patch (g) C32 showed gradual decrease in CD44 expression with NIC‐medi‐patch confirming efficacy of NIC‐GR‐PCL medi‐patch.

Article Snippet: Human melanoma cell line C32 was obtained from American Type Culture Collection (ATCC).

Techniques: Flow Cytometry, Incubation, Control, Expressing, Migration

In vivo use of e‐Medi‐Patch to evaluate the efficiency in xenograft melanoma nude mouse model. (a) Timeline of experimental procedure with schematic of ‐mouse model. (b) Variation in tumor volume from the group of animals without and with treatment of e‐Medi‐Patch. A representative animal (c) during and (d) after, application of e‐Medi Patch treatment. Change in the impedance values (ΔZ) of the e‐Medi‐Patch after 3 min of treatment on the (e) left side tumor (f) right side tumor. Control indicates animals (N = 2) not treated with e‐Medi‐Patch. Treated (no drug) indicate animals (N = 2) treated with e‐Patch without drug (niclosamide). Treated (drug) indicates animals (N = 3) treated with e‐Medi‐Patch with drug (niclosamide)‐. p value of < 0.05 has been represented as * and > 0.05 as ns. (g) Representative H&E‐stained sections of tissues collected from control animals and (h) e‐Medi‐Patch treated groups. Tissues were collected from C32 cell xenograft tumor, kidney, heart, spleen, liver and lungs. Arrow heads represent loss of tissue integrity in e‐Medi‐Patch treated niclosamide. (Scale: 2 µm).

Journal: Advanced Healthcare Materials

Article Title: Electro‐Stimulated Graphene‐Polymer Nanocomposites Enable Wearable Patches With Feedback‐Controlled Drug Release

doi: 10.1002/adhm.202505894

Figure Lengend Snippet: In vivo use of e‐Medi‐Patch to evaluate the efficiency in xenograft melanoma nude mouse model. (a) Timeline of experimental procedure with schematic of ‐mouse model. (b) Variation in tumor volume from the group of animals without and with treatment of e‐Medi‐Patch. A representative animal (c) during and (d) after, application of e‐Medi Patch treatment. Change in the impedance values (ΔZ) of the e‐Medi‐Patch after 3 min of treatment on the (e) left side tumor (f) right side tumor. Control indicates animals (N = 2) not treated with e‐Medi‐Patch. Treated (no drug) indicate animals (N = 2) treated with e‐Patch without drug (niclosamide). Treated (drug) indicates animals (N = 3) treated with e‐Medi‐Patch with drug (niclosamide)‐. p value of < 0.05 has been represented as * and > 0.05 as ns. (g) Representative H&E‐stained sections of tissues collected from control animals and (h) e‐Medi‐Patch treated groups. Tissues were collected from C32 cell xenograft tumor, kidney, heart, spleen, liver and lungs. Arrow heads represent loss of tissue integrity in e‐Medi‐Patch treated niclosamide. (Scale: 2 µm).

Article Snippet: Human melanoma cell line C32 was obtained from American Type Culture Collection (ATCC).

Techniques: In Vivo, Control, Staining

Co-cultures of C. reinhardtii and C32 tumor cells. Microalgae were added to sub-confluent C32 seeded plates and left for 24 h in standard culture conditions. A general view of the co-culture is shown in ( A , left ), with arrowheads showing cells that are detaching from the plate. A significantly lower surface area was found to be covered by C32 cells in co-culture conditions ( A , right ). A cytoskeleton analysis of the cells shows that actin fibers increased in the periphery of the cells when co-cultured ( B , upper right ). The actin fiber directionality analysis is color coded, and results show significantly increased fiber coherency in co-culture conditions ( B , lower right ). All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars represent 50 µm in ( A ) and 20 µm in ( B ). p ≤ 0.05 was considered as significant using a Mann–Whitney test. * = p ≤ 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

doi: 10.3390/ijms232113550

Figure Lengend Snippet: Co-cultures of C. reinhardtii and C32 tumor cells. Microalgae were added to sub-confluent C32 seeded plates and left for 24 h in standard culture conditions. A general view of the co-culture is shown in ( A , left ), with arrowheads showing cells that are detaching from the plate. A significantly lower surface area was found to be covered by C32 cells in co-culture conditions ( A , right ). A cytoskeleton analysis of the cells shows that actin fibers increased in the periphery of the cells when co-cultured ( B , upper right ). The actin fiber directionality analysis is color coded, and results show significantly increased fiber coherency in co-culture conditions ( B , lower right ). All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars represent 50 µm in ( A ) and 20 µm in ( B ). p ≤ 0.05 was considered as significant using a Mann–Whitney test. * = p ≤ 0.05.

Article Snippet: The C32 human melanoma cell line was obtained from ATCC (CRL-1585).

Techniques: Co-Culture Assay, Cell Culture, MANN-WHITNEY

Effect of illumination in co-cultures of C. reinhardtii and C32 tumor cells. Cells were co-cultured for 24 h in the absence or presence of light. As shown by an MTT assay, regardless of the presence of light, the mitochondrial activity of C32 cells decreased significantly in co-cultures ( A , left ), while cell death did not vary amongst groups but was slightly increased from control ( A , right ). Similarly, light did not affect viability nor proliferation capacity of the microalgae in co-cultures; however, its mortality was significantly lower when compared to control monocultures in cell media ( B ). The effect of light on hypoxia was evaluated ( C ) by HIF-1α expression. Cells were grown in normoxia (I), incubated with CoCl (II) or grown in hypoxia (III). HIF-1α decreased in illuminated hypoxic co-cultures (IV) when compared to hypoxic co-cultures in the dark (V). All assays were performed in at least three independent experiments and data are presented as average + SE. The scale bar represents 1 mm in ( B ). p ≤ 0.05 was considered as significant by using a two-way ANOVA test in an MTT viability assay, a Mann–Whitney test in an LDH release assay, and one-way ANOVA in B and C. * = p ≤ 0.05, ns = not significant.

Journal: International Journal of Molecular Sciences

Article Title: Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

doi: 10.3390/ijms232113550

Figure Lengend Snippet: Effect of illumination in co-cultures of C. reinhardtii and C32 tumor cells. Cells were co-cultured for 24 h in the absence or presence of light. As shown by an MTT assay, regardless of the presence of light, the mitochondrial activity of C32 cells decreased significantly in co-cultures ( A , left ), while cell death did not vary amongst groups but was slightly increased from control ( A , right ). Similarly, light did not affect viability nor proliferation capacity of the microalgae in co-cultures; however, its mortality was significantly lower when compared to control monocultures in cell media ( B ). The effect of light on hypoxia was evaluated ( C ) by HIF-1α expression. Cells were grown in normoxia (I), incubated with CoCl (II) or grown in hypoxia (III). HIF-1α decreased in illuminated hypoxic co-cultures (IV) when compared to hypoxic co-cultures in the dark (V). All assays were performed in at least three independent experiments and data are presented as average + SE. The scale bar represents 1 mm in ( B ). p ≤ 0.05 was considered as significant by using a two-way ANOVA test in an MTT viability assay, a Mann–Whitney test in an LDH release assay, and one-way ANOVA in B and C. * = p ≤ 0.05, ns = not significant.

Article Snippet: The C32 human melanoma cell line was obtained from ATCC (CRL-1585).

Techniques: Cell Culture, MTT Assay, Activity Assay, Control, Expressing, Incubation, MTT Viability Assay, MANN-WHITNEY, Lactate Dehydrogenase Assay

Establishment of a 3D tumor model. C32 cells were seeded in a collagen-GAG scaffold; 48 h post seeding, a significant increase in metabolic activity was quantified by MTT assays ( A ). 3D tumor model was cut into 5 µm slides and cell distribution was visualized by fluorescence microscopy ( B ). A heatmap ( C , upper left ) shows the cell distribution in the scaffold, showing a non-homogeneous internal distribution. Detailed analysis of the sections ( C , right ) shows that cells concentrate in the central sections of the scaffold. LCSM analysis of the seeded scaffolds shows tumor-like structures ( D , left ). Arrowheads in the magnified areas show cell–cell interactions and cell proliferation ( D , upper right ) and cell–matrix interactions ( D , lower right ). All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars represent 1 mm in ( A , B ) and 25 µm in ( D ). p ≤ 0.05 was considered as significant using a one-way ANOVA test. * = p ≤ 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

doi: 10.3390/ijms232113550

Figure Lengend Snippet: Establishment of a 3D tumor model. C32 cells were seeded in a collagen-GAG scaffold; 48 h post seeding, a significant increase in metabolic activity was quantified by MTT assays ( A ). 3D tumor model was cut into 5 µm slides and cell distribution was visualized by fluorescence microscopy ( B ). A heatmap ( C , upper left ) shows the cell distribution in the scaffold, showing a non-homogeneous internal distribution. Detailed analysis of the sections ( C , right ) shows that cells concentrate in the central sections of the scaffold. LCSM analysis of the seeded scaffolds shows tumor-like structures ( D , left ). Arrowheads in the magnified areas show cell–cell interactions and cell proliferation ( D , upper right ) and cell–matrix interactions ( D , lower right ). All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars represent 1 mm in ( A , B ) and 25 µm in ( D ). p ≤ 0.05 was considered as significant using a one-way ANOVA test. * = p ≤ 0.05.

Article Snippet: The C32 human melanoma cell line was obtained from ATCC (CRL-1585).

Techniques: Activity Assay, Fluorescence, Microscopy

Morphological analysis of a photosynthetic 3D tumor model. C32 cells were seeded in a collagen-GAG scaffold and cultured for 48 h, with microalgae subsequently added. Overview images of the scaffolds show a generally homogeneous distribution of the microalgae ( A ). MTT analysis shows significantly lower metabolic activity in co-cultured scaffolds compared to control scaffolds ( B ). SEM images of 2-h co-cultured scaffolds show tumor cells in direct contact with microalgae ( C ). The top-view arrowheads ( C , left ) show microalgae in between collagen sheets, while side-view arrowheads ( C , right ) indicate microalgae interacting with cancer cells. Detailed LCSM images of 2-h co-cultured matrices ( D ) show direct physical contact between C32 cells (DAPI/blue) and microalgae (chlorophyll/red) ( D , left ). Right panels show magnifications of the indicated sections on the left panel. All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars represent 1 mm for ( A ), 20 µm for ( C ), and 50 µm for ( D ). p ≤ 0.05 was considered as significant using a Mann–Whitney test. * = p ≤ 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

doi: 10.3390/ijms232113550

Figure Lengend Snippet: Morphological analysis of a photosynthetic 3D tumor model. C32 cells were seeded in a collagen-GAG scaffold and cultured for 48 h, with microalgae subsequently added. Overview images of the scaffolds show a generally homogeneous distribution of the microalgae ( A ). MTT analysis shows significantly lower metabolic activity in co-cultured scaffolds compared to control scaffolds ( B ). SEM images of 2-h co-cultured scaffolds show tumor cells in direct contact with microalgae ( C ). The top-view arrowheads ( C , left ) show microalgae in between collagen sheets, while side-view arrowheads ( C , right ) indicate microalgae interacting with cancer cells. Detailed LCSM images of 2-h co-cultured matrices ( D ) show direct physical contact between C32 cells (DAPI/blue) and microalgae (chlorophyll/red) ( D , left ). Right panels show magnifications of the indicated sections on the left panel. All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars represent 1 mm for ( A ), 20 µm for ( C ), and 50 µm for ( D ). p ≤ 0.05 was considered as significant using a Mann–Whitney test. * = p ≤ 0.05.

Article Snippet: The C32 human melanoma cell line was obtained from ATCC (CRL-1585).

Techniques: Cell Culture, Activity Assay, Control, MANN-WHITNEY

Characterization of a photosynthetic tumor model. Microalgae were seeded into scaffolds with grown tumors and incubated for two hours under physiological conditions. A histologic analysis of the scaffolds was conducted by staining tumor cells with Hematoxylin/Eosin against Ki-67 and Caspase-3. Arrowheads indicate the presence of microalgae clusters (H/E) and positive nuclei for the corresponding stain (Ki-67 and Caspase-3). Ki-67 quantification ( A , lower left ) shows a significant increase in tumor cell proliferation when microalgae are present, while Caspase-3 quantification ( A , lower right ) shows no significant differences were detected. Oxygraphic studies of co-cultured scaffolds show that, in darkness ( B , middle ), co-cultured scaffolds consume twice as much oxygen as control scaffolds. Under light ( B , right ), control (C32) scaffolds keep consuming oxygen, while co-cultured scaffolds produce over twice the consumed amount of oxygen. All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars in ( A ) represent 100 µm. In all experiments, p ≤ 0.05 was considered as significant using a Mann–Whitney test. * = p ≤ 0.05, ns = not significant.

Journal: International Journal of Molecular Sciences

Article Title: Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

doi: 10.3390/ijms232113550

Figure Lengend Snippet: Characterization of a photosynthetic tumor model. Microalgae were seeded into scaffolds with grown tumors and incubated for two hours under physiological conditions. A histologic analysis of the scaffolds was conducted by staining tumor cells with Hematoxylin/Eosin against Ki-67 and Caspase-3. Arrowheads indicate the presence of microalgae clusters (H/E) and positive nuclei for the corresponding stain (Ki-67 and Caspase-3). Ki-67 quantification ( A , lower left ) shows a significant increase in tumor cell proliferation when microalgae are present, while Caspase-3 quantification ( A , lower right ) shows no significant differences were detected. Oxygraphic studies of co-cultured scaffolds show that, in darkness ( B , middle ), co-cultured scaffolds consume twice as much oxygen as control scaffolds. Under light ( B , right ), control (C32) scaffolds keep consuming oxygen, while co-cultured scaffolds produce over twice the consumed amount of oxygen. All assays were performed in at least three independent experiments and data are presented as average + SE. Scale bars in ( A ) represent 100 µm. In all experiments, p ≤ 0.05 was considered as significant using a Mann–Whitney test. * = p ≤ 0.05, ns = not significant.

Article Snippet: The C32 human melanoma cell line was obtained from ATCC (CRL-1585).

Techniques: Incubation, Staining, Cell Culture, Control, MANN-WHITNEY

The cell viability after 24 h determined by the MTT colorimetric assay in ( a ) melanotic melanoma A375 cells, ( b ) amelanotic melanoma C32 cells, ( c ) glioblastoma SNB-19 cells, ( d ) breast adenocarcinoma MCF-7/WT cells and ( e ) drug-resistant breast adenocarcinoma MCF-7/DX cells.

Journal: International Journal of Molecular Sciences

Article Title: Synthesis, Anticancer Activity and Molecular Docking Studies of Novel N -Mannich Bases of 1,3,4-Oxadiazole Based on 4,6-Dimethylpyridine Scaffold

doi: 10.3390/ijms231911173

Figure Lengend Snippet: The cell viability after 24 h determined by the MTT colorimetric assay in ( a ) melanotic melanoma A375 cells, ( b ) amelanotic melanoma C32 cells, ( c ) glioblastoma SNB-19 cells, ( d ) breast adenocarcinoma MCF-7/WT cells and ( e ) drug-resistant breast adenocarcinoma MCF-7/DX cells.

Article Snippet: The following cell lines were used in the study: human melanotic melanoma cell line A375 (CRL-1619TM); human amelanotic melanoma cell line C32 (CRL-1585TM); human glioblastoma SNB-19 (CRL-2219TM); two breast adenocarcinoma cell lines: sensitive MCF-7/WT and resistant MCF-7/DX; and immortalized human keratinocyte from histologically normal skin HaCaT, purchased from the American Type Culture Collection (ATCC ® ).

Techniques: Colorimetric Assay

The cell viability after 24 h exposure to ( a ) compound 5 and ( b ) compound 6 , determined by the MTT colorimetric assay in skin cancers: melanotic (A375) and amelanotic (C32) melanoma cells and human keratinocytes (HaCaT). # p ≤ 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Synthesis, Anticancer Activity and Molecular Docking Studies of Novel N -Mannich Bases of 1,3,4-Oxadiazole Based on 4,6-Dimethylpyridine Scaffold

doi: 10.3390/ijms231911173

Figure Lengend Snippet: The cell viability after 24 h exposure to ( a ) compound 5 and ( b ) compound 6 , determined by the MTT colorimetric assay in skin cancers: melanotic (A375) and amelanotic (C32) melanoma cells and human keratinocytes (HaCaT). # p ≤ 0.05.

Techniques: Colorimetric Assay

Cytotoxicity index IC 50 for compounds 5 and 6 for all tested cell lines.

Journal: International Journal of Molecular Sciences

Article Title: Synthesis, Anticancer Activity and Molecular Docking Studies of Novel N -Mannich Bases of 1,3,4-Oxadiazole Based on 4,6-Dimethylpyridine Scaffold

doi: 10.3390/ijms231911173

Figure Lengend Snippet: Cytotoxicity index IC 50 for compounds 5 and 6 for all tested cell lines.

Techniques:

Colony-forming properties of the C32, A375 and HaCaT cells after incubation with ( a ) compound 5 and ( b ) compound 6 (CFU—colony forming units expressed per mL); ( c ) clonogenic assay visualization for 5 and 6 compounds. # p ≤ 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Synthesis, Anticancer Activity and Molecular Docking Studies of Novel N -Mannich Bases of 1,3,4-Oxadiazole Based on 4,6-Dimethylpyridine Scaffold

doi: 10.3390/ijms231911173

Figure Lengend Snippet: Colony-forming properties of the C32, A375 and HaCaT cells after incubation with ( a ) compound 5 and ( b ) compound 6 (CFU—colony forming units expressed per mL); ( c ) clonogenic assay visualization for 5 and 6 compounds. # p ≤ 0.05.

Techniques: Incubation, Clonogenic Assay

The population doubling time test was performed on human keratinocytes HaCaT ( a , d ), a melanotic melanoma cell line (A375) ( b , e ) and an amelanotic melanoma cell line (C32) ( c , f ) that were incubated at a density of 300 × 10 3 /well. Cells were exposed to compounds for 24 h and 72 h at three different concentrations, 25, 50 and 100 µM for compound 5 , and 100, 200 and 300 µM for compound 6 . Control cells were maintained in a growth medium with 10% fetal bovine serum (FBS) without treatment by any compounds. The number of cells was determined by counting using KOVA Slide. The results are presented as a log2 (no. of viable cells/no. of plated cells), performed three times.

Journal: International Journal of Molecular Sciences

Article Title: Synthesis, Anticancer Activity and Molecular Docking Studies of Novel N -Mannich Bases of 1,3,4-Oxadiazole Based on 4,6-Dimethylpyridine Scaffold

doi: 10.3390/ijms231911173

Figure Lengend Snippet: The population doubling time test was performed on human keratinocytes HaCaT ( a , d ), a melanotic melanoma cell line (A375) ( b , e ) and an amelanotic melanoma cell line (C32) ( c , f ) that were incubated at a density of 300 × 10 3 /well. Cells were exposed to compounds for 24 h and 72 h at three different concentrations, 25, 50 and 100 µM for compound 5 , and 100, 200 and 300 µM for compound 6 . Control cells were maintained in a growth medium with 10% fetal bovine serum (FBS) without treatment by any compounds. The number of cells was determined by counting using KOVA Slide. The results are presented as a log2 (no. of viable cells/no. of plated cells), performed three times.

Techniques: Incubation, Control

Immunofluorescence studies of C32, A375 and HaCaT cells’ structure (60×) 24 h after the treatment with compounds 5 and 6 . DAPI (4′,6-diamidino-2-phenylindole) was used for nuclei staining (blue) and actin filaments were labeled with phallotoxin (red).

Journal: International Journal of Molecular Sciences

Article Title: Synthesis, Anticancer Activity and Molecular Docking Studies of Novel N -Mannich Bases of 1,3,4-Oxadiazole Based on 4,6-Dimethylpyridine Scaffold

doi: 10.3390/ijms231911173

Figure Lengend Snippet: Immunofluorescence studies of C32, A375 and HaCaT cells’ structure (60×) 24 h after the treatment with compounds 5 and 6 . DAPI (4′,6-diamidino-2-phenylindole) was used for nuclei staining (blue) and actin filaments were labeled with phallotoxin (red).

Techniques: Immunofluorescence, Staining, Labeling

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